Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vitro Antitumor Activities of Platycarya strobilacea Sieb et Zucc Infructescence Extracts

Liangliang Zhang^1,2 , Yongmei Wang^1,2, Man Xu¹

¹Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, National Engineering Lab. for Biomass Chemical Utilization, Key and Open Lab. of Forest Chemical Engineering, State Forestry Administration, Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042; ²Institute of New Technology of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

For correspondence:- Liangliang Zhang Email: zhll20086@163.com Tel:+862585482463

Received: 30 August 2013 Accepted: 15 March 2014 Published: 26 June 2014

Citation: Zhang L, Wang Y, Xu M. In vitro Antitumor Activities of Platycarya strobilacea Sieb et Zucc Infructescence Extracts. Trop J Pharm Res 2014; 13(6):849-854 doi: 10.4314/tjpr.v13i6.4

© 2014 The authors.
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Abstract

Purpose: To evaluate the antitumor activities of Platycarya strobilacea infructescence extracts in A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB cell lines.

Methods: The total amount of phenolics in P. strobilacea infructescence based on three solvent extracts (methanol, ethyl acetate and water) was measured using Folin-Ciocalteu reagent method. The cytotoxic effect of the various solvent extracts and tannins on five cancerous cell lines (A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB) were assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT].

Results: Ethyl acetate extract had the highest phenolic content (725.02 mg GAE/g), followed by methanol extract (627.64 mg GAE/g). Among the extracts tested, ethyl acetate extract had effective anticancer activity against A549, HepG2, HCT116 and U2OS-NKFB cells with IC₅₀ values of 40.1, 42.6, 40.1 and 73.4 µg/mL, respectively. Methanol extract had the strongest cytotoxic effect on SH-SY-5Y cell with IC₅₀ value of 31.2 µg/mL. The number of surviving cancerous cells decreased as the concentration of the ethyl acetate and methanol extracts increased.

Conclusion: The study confirms the antitumor activities of ethyl acetate and methanol extracts of P. strobilacea infructescence. Thus, the plant extracts constitute potential therapeutic materials that require further studies and development.

Keywords: Platycarya strobilacea, Antitumor, Phenolic compounds, Tannins, MTT assay